Justus CD, Anderhag P, Goins JL, Lazzaro MD (2004) Microtubules and microfilaments coordinate to direct a fountain streaming pattern in elongating conifer pollen tube tips. Planta 219:103-109

Supplemental videos of pollen tubes.

Videos are in Quicktime format. Download the free Quicktime plugin. You can contact the authors at lazzarom@cofc.edu or see the Lazzaro Lab website at www.cofc.edu/~lazzaro

Figure 2: After external application of control solution, organelles continue moving in a fountain pattern as the pollen tube elongates. This sequence runs from 21:40-23:20 (min:sec) after control application. Capture rate was 1 frame per second and is replayed here at 10 frames per second.

Figure 3: After microfilaments are disrupted by latrunculin B, organelle streaming and pollen tube growth stops. Thin tubules steadily accumulate throughout the tip. This sequence runs from 35:20-37:00 (min:sec) after external latrunculin B application.

Figure 5: After microtubules are disrupted by APM, organelle streaming is reduced and pollen tube growth stops. The organization of the tip collapses but the plasma membrane remains intact. This sequence runs from 13:20-16:40 (min:sec) after external APM application.

Figure 7: Before propyzamide, organelles stream in a fountain pattern (4:50-6:40). Propyzamide was applied at 10:00 elapsed time. After microtubules are disrupted by propyzamide, organelles streaming switches to a reverse fountain pattern that becomes more distinct over time (25:00-28:50 elapsed time).

Figure 9: After microtubules are disrupted by oryzalin, organelle streaming also switches to a reverse fountain pattern as vacuoles move into the tip. The reversal begins at the start of this sequence and becomes more distinct over time. This sequence runs from 12:40-16:00 (min:sec) after external oryzalin application.